6KRZ
Crystal structure of the human adiponectin receptor 1 D208A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-15 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.589, 119.350, 197.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.900 - 3.050 |
R-factor | 0.2182 |
Rwork | 0.216 |
R-free | 0.27310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3WXV |
RMSD bond length | 0.002 |
RMSD bond angle | 0.430 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549+SVN) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.900 | 47.900 | 3.230 |
High resolution limit [Å] | 3.048 | 9.140 | 3.048 |
Rmerge | 0.260 | 0.042 | 1.494 |
Rmeas | 0.284 | 0.047 | 1.631 |
Rpim | 0.114 | 0.020 | 0.651 |
Number of reflections | 47972 | 1943 | 7573 |
<I/σ(I)> | 7 | ||
Completeness [%] | 99.8 | 99.2 | 99 |
Redundancy | 6.1 | 5.5 | 6.2 |
CC(1/2) | 0.992 | 0.998 | 0.515 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7.5 | 293 | PEG 400, tris, lithium acetate |