6KNZ

Crystal structure of T2R-TTL-KXO1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97777
Spacegroup name	P 21 21 21
Unit cell lengths	104.928, 157.059, 182.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.232 - 2.475
R-factor	0.2206
Rwork	0.220
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iyz
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.475	2.475
Number of reflections	106839	5268
<I/σ(I)>	19.5
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.995	0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2