6KNM

Apelin receptor in complex with single domain antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-12-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	44.780, 48.390, 350.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.641 - 3.200
R-factor	0.2598
Rwork	0.258
R-free	0.30510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nc2 5vbl
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.641	46.641	3.280
High resolution limit [Å]	3.200	14.310	3.200
Rmerge	0.190	0.081	0.281
Rmeas		0.100	0.369
Number of reflections	12402	176	589
<I/σ(I)>	4.38	7.84	1.86
Completeness [%]	92.5	89.8	62.1
Redundancy	3.2	2.835	1.844
CC(1/2)	0.990	0.996	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	100 mM MES pH 6.1, 26% PEG500 DME, 125 mM MgCl2, 100 mM NaCl