6KN0

caspase-1 P20/P10 C285A in complex with human GSDMD-C domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	93
Detector technology	PIXEL
Collection date	2019-01-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	141.039, 94.894, 76.081
Unit cell angles	90.00, 116.43, 90.00

Refinement procedure

Resolution	47.447 - 2.793
R-factor	0.2457
Rwork	0.240
R-free	0.29970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kmu
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.450	2.870
High resolution limit [Å]	2.790	2.790
Rmerge	0.094	0.457
Number of reflections	21818	1526
<I/σ(I)>	9.2	2.12
Completeness [%]	97.0
Redundancy	3.32
CC(1/2)	0.993	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	150 mM KBr, 30% (w/v) polyethylene glycol mono-methyester 2000