6KM7

The structural basis for the internal interaction in RBBP5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97855
Spacegroup name	P 1
Unit cell lengths	57.571, 68.841, 72.788
Unit cell angles	99.24, 99.31, 113.81

Refinement procedure

Resolution	34.772 - 1.801
R-factor	0.1664
Rwork	0.165
R-free	0.19430
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.962
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHARP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.860
High resolution limit [Å]	1.800	3.880	1.800
Rmerge	0.085	0.061	0.421
Rmeas	0.092	0.065	0.462
Rpim	0.035	0.025	0.186
Total number of observations	583704
Number of reflections	86928	8765	8055
<I/σ(I)>	5.4
Completeness [%]	96.3	97.2	89.2
Redundancy	6.7	7.2	5.5
CC(1/2)		0.987	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.1 M HEPES-Na, pH 7.5, 2% w/v PEG 400, 2.0 M ammonium sulfate