6KK7
Structure of thermal-stabilised(M6) human GLP-1 receptor transmembrane domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2017-02-25 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.230, 71.060, 81.000 |
| Unit cell angles | 92.48, 92.61, 105.10 |
Refinement procedure
| Resolution | 40.392 - 3.100 |
| R-factor | 0.2589 |
| Rwork | 0.257 |
| R-free | 0.30320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vew |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.557 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.110 | |
| Number of reflections | 20302 | 2761 |
| <I/σ(I)> | 4.6 | 1.6 |
| Completeness [%] | 79.9 | 74.5 |
| Redundancy | 2.1 | 1.9 |
| CC(1/2) | 0.990 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 0.4-0.45 M ammonium acetate, 0.1 M sodium cacodylate, pH 6.2-6.6, 35-38% PEG400, 3% w/v aminohexanoic acid |
