6KDN
HIV-1 reverse transcriptase with Q151M/Y115F/F116Y:DNA:dGTP ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-25 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | H 3 |
| Unit cell lengths | 284.167, 284.167, 95.521 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.603 - 2.303 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xn1 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.722 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.069 | 0.888 |
| Number of reflections | 130151 | 6400 |
| <I/σ(I)> | 17.2 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | BIS-TRIS-HCL, DI-AMMONIUM HYDROGEN CITRATE, MGCL2, PEG 6000, SUCROSE, GLYCEROL |
