6K1L

E53A mutant of a putative cystathionine gamma-lyase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	59.970, 148.460, 156.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	67.105 - 2.460
R-factor	0.2084
Rwork	0.206
R-free	0.27570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nmp
RMSD bond length	0.008
RMSD bond angle	1.004
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.230	2.520
High resolution limit [Å]	2.460	2.460
Number of reflections	51467	3612
<I/σ(I)>	11.4
Completeness [%]	99.3
Redundancy	12.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M Potassium sulphate, 20% (v/v) polyethylene glycol 3350, 5 mM cysteine