6K0R
Ruvbl1-Ruvbl2 with truncated domain II in complex with phosphorylated Cordycepin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 110.304, 186.424, 235.176 |
| Unit cell angles | 90.00, 91.04, 90.00 |
Refinement procedure
| Resolution | 49.720 - 2.502 |
| R-factor | 0.23 |
| Rwork | 0.228 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xsz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 235.137 | 2.810 |
| High resolution limit [Å] | 2.502 | 2.502 |
| Rmerge | 0.107 | 1.033 |
| Rmeas | 0.120 | 1.138 |
| Rpim | 0.054 | 0.474 |
| Number of reflections | 90883 | 4542 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 89.9 | |
| Redundancy | 4.6 | |
| CC(1/2) | 0.996 | 0.159 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M HEPES-Na pH 7.5-7.6, 0.2 M MgCl2, 20-21 % PEG400 |
