6JWI
Yeast Npl4 in complex with Lys48-linked diubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97904 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.351, 103.103, 99.620 |
| Unit cell angles | 90.00, 100.40, 90.00 |
Refinement procedure
| Resolution | 48.990 - 2.550 |
| R-factor | 0.1924 |
| Rwork | 0.191 |
| R-free | 0.22570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jwh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.215 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.580 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 55810 | 2546 |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.1 | 91.3 |
| Redundancy | 19.3 | 6.1 |
| CC(1/2) | 0.504 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100mM Bicine-NaOH (pH 6.5), 18% PEG 3350, 200mM lithium sulfate |
