6JLQ
Crystal structure of human USP46-WDR48-WDR20 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 217.230, 217.230, 223.933 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.986 - 3.101 |
| R-factor | 0.2479 |
| Rwork | 0.247 |
| R-free | 0.27170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k1c |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.466 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 6.670 | 3.100 |
| Rmerge | 0.141 | 0.047 | 1.800 |
| Rmeas | 0.148 | 0.050 | 1.879 |
| Rpim | 0.041 | 0.014 | 0.509 |
| Total number of observations | 616763 | ||
| Number of reflections | 56304 | 5855 | 5580 |
| <I/σ(I)> | 9.9 | ||
| Completeness [%] | 99.0 | 96.4 | 99.9 |
| Redundancy | 11 | 10.3 | 11.1 |
| CC(1/2) | 0.999 | 0.519 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.0M Sodium phosphate monobasic monohydrate, Potassium phosphate dibasic, pH 7.2 |
