6JLQ

Crystal structure of human USP46-WDR48-WDR20 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ubiquitin carboxyl-terminal hydrolase 46	polymer	352	41262.9	1	UniProt (P62068) Pfam (PF00443) In PDB	Homo sapiens (Human)	Deubiquitinating enzyme 46,Ubiquitin thioesterase 46,Ubiquitin-specific-processing protease 46
2	B	WD repeat-containing protein 48	polymer	620	69730.6	1	UniProt (Q8TAF3) Pfam (PF00400) Pfam (PF11816) In PDB	Homo sapiens (Human)
3	C	WD repeat-containing protein 20,highly similar to WD repeat protein 20,WD repeat-containing protein 20	polymer	444	48560.9	1	UniProt (Q8TBZ3) UniProt (B3KQX8) UniProt (L8I535) Pfam (PF00400) In PDB	Homo sapiens (Human)	Protein DMR
4	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
5	A, B, C	GLYCEROL	non-polymer		92.1	8	Chemie (GOL)
6	B	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)

Sequence modifications

A: 24 - 366 (UniProt: P62068)

PDB	External Database	Details
Met 23	-	initiating methionine
Leu 367	-	expression tag
Glu 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag
His 374	-	expression tag

B: 1 - 580 (UniProt: Q8TAF3)

PDB	External Database	Details
Met -39	-	initiating methionine
Ser -38	-	expression tag
Tyr -37	-	expression tag
Tyr -36	-	expression tag
Asp -35	-	expression tag
Tyr -34	-	expression tag
Lys -33	-	expression tag
Asp -32	-	expression tag
Asp -31	-	expression tag
Asp -30	-	expression tag
Asp -29	-	expression tag
Lys -28	-	expression tag
Ser -27	-	expression tag
Gly -26	-	expression tag
Ser -25	-	expression tag
Gly -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Asp -17	-	expression tag
Tyr -16	-	expression tag
Asp -15	-	expression tag
Ile -14	-	expression tag
Pro -13	-	expression tag
Thr -12	-	expression tag
Thr -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

C: 1 - 318 (UniProt: Q8TBZ3)

PDB	External Database	Details
Met -7	-	initiating methionine
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Gly 319	-	linker
Gly 320	-	linker
Gly 321	-	linker
Gly 361	-	linker
Ser 362	-	linker

C: 363 - 488 (UniProt: B3KQX8)

PDB	External Database	Details
Gly 489	-	linker
Gly 490	-	linker
Gly 491	-	linker
Gly 492	-	linker
Ser 493	-	linker
Gly 494	-	linker
Gly 495	-	linker
Gly 496	-	linker
Gly 497	-	linker
Ser 498	-	linker
Gly 499	-	linker
Gly 500	-	linker
Gly 501	-	linker
Gly 502	-	linker
Ser 503	-	linker
Gly 504	-	linker
Gly 505	-	linker
Gly 506	-	linker
Gly 507	-	linker
Ser 508	-	linker

C: 509 - 569 (UniProt: L8I535)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	159554.4
Non-Polymers*	Number of molecules	10
	Total formula weight	897.1
All*	Total formula weight	160451.5

*Water molecules are not included.