6JE4
Crystal structure of Nme1Cas9-sgRNA-dsDNA dimer mediated by double protein inhibitor AcrIIC3 monomers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-31 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.06000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 129.199, 118.010, 323.986 |
| Unit cell angles | 90.00, 89.92, 90.00 |
Refinement procedure
| Resolution | 46.284 - 3.069 |
| R-factor | 0.2117 |
| Rwork | 0.210 |
| R-free | 0.24090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6jdq |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.837 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3247: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Rmerge | 0.133 | 0.701 |
| Rmeas | 0.150 | 0.804 |
| Rpim | 0.069 | 0.384 |
| Number of reflections | 178199 | 8728 |
| <I/σ(I)> | 9.4 | 1.6 |
| Completeness [%] | 98.5 | 97.4 |
| Redundancy | 4.3 | 3.9 |
| CC(1/2) | 0.980 | 0.508 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.8 | 289 | 1M NaCl, 0.1M citric pH 5.76, 30% PEG 600, 9.6% glycerol |
