6JE4

Crystal structure of Nme1Cas9-sgRNA-dsDNA dimer mediated by double protein inhibitor AcrIIC3 monomers

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0016787	molecular_function	hydrolase activity
A	0043571	biological_process	maintenance of CRISPR repeat elements
A	0046872	molecular_function	metal ion binding
A	0051607	biological_process	defense response to virus
E	0003676	molecular_function	nucleic acid binding
E	0003677	molecular_function	DNA binding
E	0003723	molecular_function	RNA binding
E	0004518	molecular_function	nuclease activity
E	0004519	molecular_function	endonuclease activity
E	0016787	molecular_function	hydrolase activity
E	0043571	biological_process	maintenance of CRISPR repeat elements
E	0046872	molecular_function	metal ion binding
E	0051607	biological_process	defense response to virus
K	0003676	molecular_function	nucleic acid binding
K	0003677	molecular_function	DNA binding
K	0003723	molecular_function	RNA binding
K	0004518	molecular_function	nuclease activity
K	0004519	molecular_function	endonuclease activity
K	0016787	molecular_function	hydrolase activity
K	0043571	biological_process	maintenance of CRISPR repeat elements
K	0046872	molecular_function	metal ion binding
K	0051607	biological_process	defense response to virus
O	0003676	molecular_function	nucleic acid binding
O	0003677	molecular_function	DNA binding
O	0003723	molecular_function	RNA binding
O	0004518	molecular_function	nuclease activity
O	0004519	molecular_function	endonuclease activity
O	0016787	molecular_function	hydrolase activity
O	0043571	biological_process	maintenance of CRISPR repeat elements
O	0046872	molecular_function	metal ion binding
O	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue EDO B 201

Chain	Residue
B	G98
B	C99
B	U127

---

site_id	AC2
Number of Residues	2
Details	binding site for residue PEG B 202

Chain	Residue
B	G71
B	G83

---

site_id	AC3
Number of Residues	2
Details	binding site for residue EDO E 1101

Chain	Residue
E	PHE46
E	ARG479

---

site_id	AC4
Number of Residues	1
Details	binding site for residue EDO E 1102

Chain	Residue
E	GLY932

---

site_id	AC5
Number of Residues	2
Details	binding site for residue EDO E 1103

Chain	Residue
E	GLY496
E	VAL492

---

site_id	AC6
Number of Residues	4
Details	binding site for residue EDO F 201

Chain	Residue
E	GLU845
E	THR846
F	G25
G	DG12

---

site_id	AC7
Number of Residues	3
Details	binding site for residue EDO F 202

Chain	Residue
F	G98
F	C99
F	U127

---

site_id	AC8
Number of Residues	3
Details	binding site for residue PEG F 203

Chain	Residue
F	G71
F	G83
F	U134

---

site_id	AC9
Number of Residues	3
Details	binding site for residue EDO J 201

Chain	Residue
J	GLN52
J	TYR53
J	ASP103

---

site_id	AD1
Number of Residues	2
Details	binding site for residue EDO K 1101

Chain	Residue
K	ARG479
K	PRO834

---

site_id	AD2
Number of Residues	3
Details	binding site for residue EDO K 1102

Chain	Residue
K	ARG1015
K	PHE1017
K	HOH1201

---

site_id	AD3
Number of Residues	1
Details	binding site for residue EDO K 1103

Chain	Residue
K	GLY496

---

site_id	AD4
Number of Residues	2
Details	binding site for residue EDO K 1104

Chain	Residue
K	PHE592
K	ASP598

---

site_id	AD5
Number of Residues	5
Details	binding site for residue EDO L 201

Chain	Residue
K	GLU845
K	THR846
L	G25
L	U26
M	DG12

---

site_id	AD6
Number of Residues	2
Details	binding site for residue EDO L 202

Chain	Residue
L	C92
L	G129

---

site_id	AD7
Number of Residues	2
Details	binding site for residue EDO L 203

Chain	Residue
L	U14
M	DT23

---

site_id	AD8
Number of Residues	3
Details	binding site for residue EDO L 204

Chain	Residue
L	A30
L	G31
L	U55

---

site_id	AD9
Number of Residues	3
Details	binding site for residue EDO L 205

Chain	Residue
L	G98
L	C99
L	U127

---

site_id	AE1
Number of Residues	1
Details	binding site for residue EDO O 1101

Chain	Residue
O	ARG479

---

site_id	AE2
Number of Residues	1
Details	binding site for residue EDO O 1102

Chain	Residue
O	TYR272

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	620
Details	Domain: {"description":"HNH Cas9-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU01085","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	Active site: {"description":"For RuvC-like nuclease domain","evidences":[{"source":"HAMAP-Rule","id":"MF_01480","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	Active site: {"description":"Proton acceptor for HNH nuclease domain","evidences":[{"source":"HAMAP-Rule","id":"MF_01480","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_01480","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---