6JE4
Crystal structure of Nme1Cas9-sgRNA-dsDNA dimer mediated by double protein inhibitor AcrIIC3 monomers
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, F, K, O (A, E, K, O) | CRISPR-associated endonuclease Cas9 | polymer | 1083 | 124589.9 | 4 | UniProt (C9X1G5) Pfam (PF13395) Pfam (PF18541) | Neisseria meningitidis 8013 | |
| 2 | B, G, L, P (C, G, M, Q) | target DNA strand | polymer | 35 | 10836.0 | 4 | synthetic construct | ||
| 3 | C, H, M, Q (D, H, N, R) | non-target DNA strand | polymer | 35 | 10858.0 | 4 | synthetic construct | ||
| 4 | D, I, N, R (B, F, L, P) | sgRNA | polymer | 135 | 43059.4 | 4 | synthetic construct | ||
| 5 | E, J, S, T (I, J, S, T) | AcrIIC3 | polymer | 117 | 13457.4 | 4 | UniProt (A0A3E2QDI5) UniProt (by SIFTS) (A0A425B395) | Neisseria meningitidis 8013 | |
| 6 | AA, CA, DA, EA, FA... (F, J, K, L, O...) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 18 | Chemie (EDO) | |||
| 7 | BA, HA, V (F, K, B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 3 | Chemie (PEG) | |||
| 8 | AB, BB, CB, DB, PA... (O, Q, P, S, A...) | water | water | 18.0 | 63 | Chemie (HOH) |
Sequence modifications
A, E, K, O: 1 - 1082 (UniProt: C9X1G5)
I, J, S, T: 1 - 116 (UniProt: A0A3E2QDI5)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 16 | Asp 16 | engineered mutation |
| Ala 588 | His 588 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 20 |
| Total formula weight | 811202.8 | |
| Non-Polymers* | Number of molecules | 21 |
| Total formula weight | 1435.6 | |
| All* | Total formula weight | 812638.4 |
