6JDQ

Crystal structure of Nme1Cas9 in complex with sgRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	72.080, 122.452, 238.074
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.914 - 2.950
R-factor	0.2571
Rwork	0.255
R-free	0.28960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6jdv
RMSD bond length	0.016
RMSD bond angle	1.509
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3247: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.000
High resolution limit [Å]	2.950	2.950
Rmerge	0.097
Rmeas	0.104
Rpim	0.039	0.455
Number of reflections	45932	2270
<I/σ(I)>	19.9	1.8
Completeness [%]	100.0	100
Redundancy	7.3	7.3
CC(1/2)	0.973	0.619

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	0.1M tris pH 8.5, 0.12M K2SO4, 18.5% PEG 6000