6J4Q
Structural basis of tubulin detyrosination by vasohibins-SVBP enzyme complex and functional implications
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-23 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 117.177, 117.177, 209.044 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.307 - 2.700 |
R-factor | 0.1868 |
Rwork | 0.183 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6j4o |
RMSD bond length | 0.010 |
RMSD bond angle | 1.192 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.090 | 1.767 |
Rpim | 0.030 | 0.585 |
Number of reflections | 51721 | 5049 |
<I/σ(I)> | 25.6 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.9 | 10 |
CC(1/2) | 0.991 | 0.621 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 293 | V2c-SVBP was mixed with the inhibitor TPCK at a mole ratio of 1:5 and incubated on ice for 30 min. Then, the resulting co-complex of V2c-SVBP-TPCK was crystallized in 1.0 M sodium malonate, pH 7.0. |