6IUR
A phosphatase complex STRN3-PP2Aa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.094, 201.743, 116.055 |
| Unit cell angles | 90.00, 106.29, 90.00 |
Refinement procedure
| Resolution | 44.068 - 3.330 |
| R-factor | 0.2005 |
| Rwork | 0.199 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i5l |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.864 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.449 |
| High resolution limit [Å] | 3.330 | 3.330 |
| Rmerge | 0.116 | 1.283 |
| Rmeas | 0.142 | |
| Rpim | 0.081 | 0.897 |
| Number of reflections | 67955 | 3368 |
| <I/σ(I)> | 12.46 | |
| Completeness [%] | 99.0 | 99.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1M BIS-TRIS propane pH 9.0, 8.5% PEG20000 |
