6IS0

Crystal structure of the zebrafish cap-specific adenosine methyltransferase bound to SAH and m7G-capped RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	74.219, 84.035, 92.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.736 - 1.800
R-factor	0.1789
Rwork	0.177
R-free	0.21450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6irx
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.740	47.740	1.840
High resolution limit [Å]	1.800	9.000	1.800
Rmerge	0.050	0.039	0.576
Rmeas	0.054	0.042	0.622
Rpim	0.021	0.016	0.234
Total number of observations	364594
Number of reflections	54004	521	3128
<I/σ(I)>	19
Completeness [%]	99.1	99.6	98.3
Redundancy	6.8	6.1	6.9
CC(1/2)	0.999	0.998	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	9% PEG 3350, 0.1M potassium thiocyanate, 0.05M bis-Tris propane