6IRV

Crystal structure of the human cap-specific adenosine methyltransferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-12
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	70.699, 120.309, 156.831
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.125 - 2.700
R-factor	0.2162
Rwork	0.214
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6irx
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.125	48.120	2.820
High resolution limit [Å]	2.700	9.350	2.700
Rmerge	0.110	0.040	0.751
Rmeas	0.119	0.044	0.816
Rpim	0.046	0.018	0.316
Number of reflections	37583	1018	4535
<I/σ(I)>	10.6
Completeness [%]	100.0	99.4	100
Redundancy	6.7	5.8	6.6
CC(1/2)	0.998	0.998	0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	10% PEG 3350, 0.4M sodium nitrate, 0.1M bis-Tris propane