6IM9

MDM2 bound CueO-PM2 sensor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	83.636, 83.636, 240.171
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.920 - 3.300
R-factor	0.1801
Rwork	0.177
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1KV7 and 5WTS
RMSD bond length	0.011
RMSD bond angle	1.215
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	3.380
High resolution limit [Å]	3.300	8.090	3.300
Rmerge	0.046	0.019	0.282
Rmeas	0.052	0.021	0.326
Rpim	0.023	0.009	0.159
Total number of observations	77012
Number of reflections	15209	1129	850
<I/σ(I)>	14.1
Completeness [%]	98.7	98.8	85.8
Redundancy	5.1	4.8	3.4
CC(1/2)		0.999	0.961

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	4M sodium formate