6IJN

The D295N mutant of the N6-methyl-AMP deaminase from Arabidopsis thaliana complexed with N6m-AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	49.912, 80.086, 86.792
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.268 - 1.660
R-factor	0.1487
Rwork	0.147
R-free	0.17410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ada
RMSD bond length	0.009
RMSD bond angle	1.065
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.720
High resolution limit [Å]	1.660	1.660
Rmerge	0.103	0.471
Number of reflections	42279	4139
<I/σ(I)>	20.5	5.1
Completeness [%]	99.7	99.3
Redundancy	12.6	13
CC(1/2)	0.998	0.959

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	32% PEG 3350, 0.1 M NaCl, 0.1 M Tris-HCl (pH 9.0).