6IHC
Crystal structure of (3R)-Hydroxyacyl-Acyl Carrier Protein Dehydratase(FabZ) Y100A mutant in complex with holo-ACP from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 185 |
Detector technology | IMAGE PLATE |
Collection date | 2016-09-07 |
Detector | MACSCIENCE DIP100S |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 272.528, 76.900, 72.991 |
Unit cell angles | 90.00, 99.23, 90.00 |
Refinement procedure
Resolution | 45.644 - 2.400 |
R-factor | 0.1774 |
Rwork | 0.175 |
R-free | 0.21380 |
RMSD bond length | 0.009 |
RMSD bond angle | 0.975 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 56174 | |
<I/σ(I)> | 14.9 | |
Completeness [%] | 96.1 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20% PEG 3350, 0.05M tri-Sodium citrate dehydrate |