6IF3

Complex structure of Rab35 and its effector ACAP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	44.741, 53.468, 144.008
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.049 - 1.500
R-factor	0.1738
Rwork	0.172
R-free	0.20510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tw8 3jue
RMSD bond length	0.011
RMSD bond angle	1.255
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.530
High resolution limit [Å]	1.500	4.070	1.500
Rmerge	0.117	0.077	0.584
Rmeas	0.122	0.080	0.636
Rpim	0.035	0.023	0.239
Total number of observations	570145
Number of reflections	54675	3077	1913
<I/σ(I)>	5.2
Completeness [%]	96.7	99.9	68.8
Redundancy	10.4	12.1	4.9
CC(1/2)		0.997	0.801

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	Potassium sodium tartrate, PEG 3350