6IAN
T. brucei IFT22/74/81 GTP-bound crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97899 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.860, 228.300, 115.710 |
Unit cell angles | 90.00, 96.76, 90.00 |
Refinement procedure
Resolution | 80.982 - 3.200 |
R-factor | 0.2431 |
Rwork | 0.241 |
R-free | 0.27970 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.058 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.000 | 3.400 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.210 | 4.110 |
Number of reflections | 113747 | 18991 |
<I/σ(I)> | 11.5 | 0.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.3 | |
CC(1/2) | 0.999 | 0.395 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 277 | 15% (v/v) glycerol, 7.5% (w/v) PEG4000, 100 mM HEPES pH 7.5 |