6I9C

Structure of the OTU domain of OTULIN G281R mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	110
Detector technology	PIXEL
Collection date	2017-08-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97624
Spacegroup name	P 21 21 21
Unit cell lengths	43.260, 72.437, 93.732
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.316 - 1.770
R-factor	0.1632
Rwork	0.162
R-free	0.17860
RMSD bond length	0.006
RMSD bond angle	0.774
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	72.440	1.810
High resolution limit [Å]	1.770	1.770
Rmerge	0.058	0.840
Rmeas	0.063	0.913
Rpim	0.025	0.354
Total number of observations	189022
Number of reflections	29060	1655
<I/σ(I)>	16.3
Completeness [%]	98.6	100
Redundancy	6.5	6.6
CC(1/2)	0.999	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	100 mM HEPES pH 7.4, 18 % (w/v) PEG 6,000, 1M LiCl