6I3F
Crystal structure of the complex of human angiotensinogen and renin at 2.55 Angstrom
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 124.119, 124.119, 260.911 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 62.010 - 2.550 |
| R-factor | 0.20761 |
| Rwork | 0.206 |
| R-free | 0.23392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2WXW and 2BKS |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.192 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.060 | 2.650 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.209 | 1.250 |
| Rmeas | 0.225 | 1.350 |
| Number of reflections | 39602 | 4393 |
| <I/σ(I)> | 10.2 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.7 | 12.9 |
| CC(1/2) | 0.998 | 0.674 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 1.76M Ammonium sulfate 0.1M Tris, pH7.6 |
