6I3F
Crystal structure of the complex of human angiotensinogen and renin at 2.55 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-01 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.987 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 124.119, 124.119, 260.911 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 62.010 - 2.550 |
R-factor | 0.20761 |
Rwork | 0.206 |
R-free | 0.23392 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2WXW and 2BKS |
RMSD bond length | 0.002 |
RMSD bond angle | 1.192 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.060 | 2.650 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.209 | 1.250 |
Rmeas | 0.225 | 1.350 |
Number of reflections | 39602 | 4393 |
<I/σ(I)> | 10.2 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.7 | 12.9 |
CC(1/2) | 0.998 | 0.674 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | 1.76M Ammonium sulfate 0.1M Tris, pH7.6 |