6I1K
Crystal structure of catalytically inactive FnCas12a in complex with a crRNA guide and a dsDNA target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.172, 141.681, 89.478 |
| Unit cell angles | 90.00, 97.43, 90.00 |
Refinement procedure
| Resolution | 47.612 - 2.650 |
| R-factor | 0.249 |
| Rwork | 0.248 |
| R-free | 0.26370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nfv |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.613 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.612 | 2.745 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 58701 | |
| <I/σ(I)> | 17.86 | 0.96 |
| Completeness [%] | 99.6 | 98.67 |
| Redundancy | 6.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris Propane (BTP), pH 6.5) 0.2 M KSCN 17.5 % polyethylene glycol 3,400 |
