Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6I1K

Crystal structure of catalytically inactive FnCas12a in complex with a crRNA guide and a dsDNA target

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004530	molecular_function	deoxyribonuclease I activity
A	0006259	biological_process	DNA metabolic process
A	0016787	molecular_function	hydrolase activity
A	0016829	molecular_function	lyase activity
A	0051607	biological_process	defense response to virus

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue MG A 1401

Chain	Residue
A	ASP792
A	ARG800
A	HOH1502
A	HOH1507
B	U-4
B	A-3
B	HOH201
B	HOH203

site_id	AC2
Number of Residues	6
Details	binding site for residue MG A 1402

Chain	Residue
A	HOH1519
A	HOH1524
A	HOH1530
A	HOH1545
B	HOH204
A	HOH1517

site_id	AC3
Number of Residues	6
Details	binding site for residue MG A 1403

Chain	Residue
A	ASN700
A	HOH1529
D	HOH101
D	HOH103
D	HOH105
D	HOH106

site_id	AC4
Number of Residues	6
Details	binding site for residue MG A 1404

Chain	Residue
A	GLU827
A	HOH1501
A	HOH1516
A	HOH1536
A	HOH1539
A	HOH1540

site_id	AC5
Number of Residues	6
Details	binding site for residue MG A 1405

Chain	Residue
A	LEU66
A	SER67
A	VAL69
A	TYR248
A	ASN270
A	HOH1512

site_id	AC6
Number of Residues	7
Details	binding site for residue MG A 1406

Chain	Residue
A	ASN602
A	THR604
A	ASP616
A	LYS635
A	HOH1521
D	HOH102
D	HOH104

site_id	AC7
Number of Residues	6
Details	binding site for residue MG A 1407

Chain	Residue
A	HOH1527
A	HOH1528
A	HOH1532
A	HOH1533
A	HOH1546
B	HOH216

site_id	AC8
Number of Residues	4
Details	binding site for residue MG A 1408

Chain	Residue
A	PHE153
A	ASN156
A	ILE159
A	THR160

site_id	AC9
Number of Residues	3
Details	binding site for residue MG A 1409

Chain	Residue
A	TRP175
A	GLU601
A	SER603

site_id	AD1
Number of Residues	4
Details	binding site for residue EDO A 1410

Chain	Residue
A	GLU756
A	ASN759
A	GLN760
A	LYS889

site_id	AD2
Number of Residues	2
Details	binding site for residue EDO A 1411

Chain	Residue
A	SER12
A	PHE888

site_id	AD3
Number of Residues	6
Details	binding site for residue EDO A 1412

Chain	Residue
A	LYS32
A	GLY35
A	LEU36
A	ILE37
A	LEU38
A	LYS435

site_id	AD4
Number of Residues	5
Details	binding site for residue EDO A 1413

Chain	Residue
A	ASN305
A	LYS317
A	LYS320
C	DG-17
C	DA-16

site_id	AD5
Number of Residues	4
Details	binding site for residue EDO A 1414

Chain	Residue
A	ARG18
A	LYS595
A	HIS881
B	U-15

site_id	AD6
Number of Residues	6
Details	binding site for residue MG B 101

Chain	Residue
A	HOH1525
A	HOH1543
B	HOH202
B	HOH205
B	HOH208
B	HOH213

site_id	AD7
Number of Residues	6
Details	binding site for residue MG B 102

Chain	Residue
A	HOH1534
A	HOH1541
B	A6
B	HOH209
B	HOH212
B	HOH214

site_id	AD8
Number of Residues	2
Details	binding site for residue EDO B 103

Chain	Residue
B	U4
B	U5

site_id	AD9
Number of Residues	3
Details	binding site for residue EDO B 104

Chain	Residue
B	A8
B	A9
B	EDO105

site_id	AE1
Number of Residues	3
Details	binding site for residue EDO B 105

Chain	Residue
B	G10
B	EDO104
B	EDO107

site_id	AE2
Number of Residues	3
Details	binding site for residue EDO B 106

Chain	Residue
B	A19
B	U20
B	HOH204

site_id	AE3
Number of Residues	3
Details	binding site for residue EDO B 107

Chain	Residue
B	G10
B	G11
B	EDO105

site_id	AE4
Number of Residues	1
Details	binding site for residue EDO B 108

Chain	Residue
B	A7

site_id	AE5
Number of Residues	1
Details	binding site for residue EDO C 101

Chain	Residue
C	DT-1

site_id	AE6
Number of Residues	2
Details	binding site for residue EDO C 102

Chain	Residue
C	DT-14
C	DA-15

site_id	AE7
Number of Residues	4
Details	binding site for residue EDO C 103

Chain	Residue
C	DG4
C	DG5
C	DA6
D	DT-6

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	314
Details	Region: {"description":"Recognition domain 1","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	8
Details	Region: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	4
Details	Region: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	3
Details	Region: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	251
Details	Region: {"description":"Recognition domain 2","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	3
Details	Region: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	8
Details	Region: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	70
Details	Region: {"description":"Wedge region 2","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	17
Details	Region: {"description":"LKL, important for PAM recognition and DNA unwinding","evidences":[{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	99
Details	Region: {"description":"PAM-interacting domain (PI)","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	6
Details	Region: {"description":"Binds DNA protospacer adjacent motif (PAM) on target DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	12
Details	Region: {"description":"Binds single-strand non-target DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	8
Details	Region: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	60
Details	Region: {"description":"RuvC-I","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	17
Details	Region: {"description":"Bridge helix","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI16
Number of Residues	106
Details	Region: {"description":"RuvC-II","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI17
Number of Residues	45
Details	Region: {"description":"RuvC-III","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000303"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000303"}]}

Chain

Residue

Details

site_id	SWS_FT_FI18
Number of Residues	3
Details	Active site: {"description":"For pre-crRNA processing","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI19
Number of Residues	3
Details	Active site: {"description":"For DNase activity of RuvC domain","evidences":[{"source":"PubMed","id":"26422227","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI20
Number of Residues	1
Details	Site: {"description":"Binds crRNA alone and in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI21
Number of Residues	1
Details	Site: {"description":"Binds target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI22
Number of Residues	1
Details	Site: {"description":"Binds crRNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI23
Number of Residues	3
Details	Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI24
Number of Residues	1
Details	Site: {"description":"Caps the crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI25
Number of Residues	1
Details	Site: {"description":"Binds DNA protospacer adjacent motif (PAM)","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI26
Number of Residues	1
Details	Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI27
Number of Residues	1
Details	Site: {"description":"Binds PAM","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI28
Number of Residues	2
Details	Site: {"description":"Binds Target strand DNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI29
Number of Residues	1
Details	Site: {"description":"Binds Target strand DNA; via amide nitrogen","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI30
Number of Residues	1
Details	Site: {"description":"Binds crRNA","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI31
Number of Residues	1
Details	Site: {"description":"Stabilizes transition state for pre-crRNA processing","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI32
Number of Residues	2
Details	Site: {"description":"Binds DNA in crRNA-target DNA heteroduplex","evidences":[{"source":"PubMed","id":"28431230","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"28562584","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)