6I1K
Crystal structure of catalytically inactive FnCas12a in complex with a crRNA guide and a dsDNA target
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | CRISPR-associated endonuclease Cas12a | polymer | 1302 | 152282.2 | 1 | UniProt (A0Q7Q2) Pfam (PF18501) Pfam (PF21918) Pfam (PF22222) Pfam (PF18516) Pfam (PF18510) In PDB | Francisella tularensis subsp. novicida (strain U112) | CRISPR-associated endonuclease Cpf1,FnCas12a,FnCpf1 |
| 2 | B | crRNA (40-MER) | polymer | 40 | 12754.5 | 1 | synthetic construct | ||
| 3 | C | DNA target strand (38-MER) | polymer | 38 | 11666.5 | 1 | synthetic construct | ||
| 4 | D | DNA non-target strand (33-MER) | polymer | 38 | 11617.5 | 1 | synthetic construct | ||
| 5 | A, B | MAGNESIUM ION | non-polymer | 24.3 | 11 | Chemie (MG) | |||
| 6 | C, A, B | 1,2-ETHANEDIOL | non-polymer | 62.1 | 14 | Chemie (EDO) | |||
| 7 | water | water | 18.0 | 71 | Chemie (HOH) |
Sequence modifications
A: 2 - 1300 (UniProt: A0Q7Q2)
| PDB | External Database | Details |
|---|---|---|
| Ser -1 | - | expression tag |
| Asn 0 | - | expression tag |
| Ala 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 188320.8 | |
| Non-Polymers* | Number of molecules | 25 |
| Total formula weight | 1136.3 | |
| All* | Total formula weight | 189457.1 |
