6HZV
HUMAN JAK3 IN COMPLEX WITH LASW959 PROTEIN IN COMPLEX WITH LIGAND
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-12-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.072, 112.814, 104.466 |
| Unit cell angles | 90.00, 98.30, 90.00 |
Refinement procedure
| Resolution | 43.230 - 2.460 |
| R-factor | 0.2239 |
| Rwork | 0.223 |
| R-free | 0.27533 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yvj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.927 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.2.0005) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.230 | 2.680 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.118 | 0.767 |
| Number of reflections | 47359 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 30% PEG3350, 0.1M BIS-TRIS, 0.2M MgCl, pH 6.0 |
