6HXW
structure of human CD73 in complex with antibody IPH53
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98004 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 195.347, 209.429, 65.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.840 - 2.780 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.210 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.840 | 2.930 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.122 | 0.795 |
| Rmeas | 0.129 | |
| Rpim | 0.041 | 0.265 |
| Number of reflections | 68010 | 9400 |
| <I/σ(I)> | 5.5 | 1 |
| Completeness [%] | 99.5 | 95.2 |
| Redundancy | 9.3 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.1M succinic acid pH7.0 15% (w/v) PEG 3350 |
