6HT5
Oct4/Sox2:UTF1 structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 63 |
| Unit cell lengths | 165.170, 165.170, 39.780 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.680 - 3.451 |
| R-factor | 0.205 |
| Rwork | 0.201 |
| R-free | 0.24840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o4x |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.647 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.620 | 3.600 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.115 | 0.998 |
| Number of reflections | 8480 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 100 mM 4-morpholineethanesulfonic acid at pH 6.0, 33% v/v 2-methyl-2,4-pentanediol |
