6HKS
Crystal structure of the PTPN3 PDZ domain bound to the HPV16 E6 oncoprotein C-terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.978549599648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.620, 77.430, 130.030 |
| Unit cell angles | 90.00, 90.14, 90.00 |
Refinement procedure
| Resolution | 43.343 - 2.194 |
| R-factor | 0.196735201568 |
| Rwork | 0.194 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ez0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.938 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.620 | 2.230 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmeas | 0.140 | |
| Number of reflections | 47024 | |
| <I/σ(I)> | 8.17 | 1.11 |
| Completeness [%] | 99.1 | 95.7 |
| Redundancy | 6.9 | 6.5 |
| CC(1/2) | 0.996 | 0.726 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% w/v PEG 3350 0.2 mM KI pH 7 |
