6HJX

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) 7'C pore mutant (L238C) in complex with nanobody 72

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-01
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.91958
Spacegroup name	P 1 21 1
Unit cell lengths	96.251, 156.277, 102.496
Unit cell angles	90.00, 102.88, 90.00

Refinement procedure

Resolution	47.587 - 2.500
R-factor	0.2063
Rwork	0.204
R-free	0.24510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2yoe
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.840	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.067	0.948
Rmeas	0.079	1.128
Rpim	0.042	0.607
Number of reflections	101800	14853
<I/σ(I)>	14.8	1.3
Completeness [%]	99.9
Redundancy	3.5
CC(1/2)	0.999	0.475

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MES (pH 6.5) and 30% PEG400