6HIL

X-ray structure of TEAD1(Y421H mutant) complexed with YAP(wildtype): Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	40.125, 46.544, 144.337
Unit cell angles	98.61, 90.85, 108.14

Refinement procedure

Resolution	19.900 - 2.300
R-factor	0.2437
Rwork	0.242
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kys
RMSD bond length	0.007
RMSD bond angle	1.012
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.046	0.568
Rmeas	0.065	0.804
Rpim	0.100	0.702
Total number of observations	74068
Number of reflections	41611
<I/σ(I)>	10.9	1.2
Completeness [%]	95.5	95.4
Redundancy	1.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	22% (w/v) PEG3350, 0.2M Na Malonate