6HIK
X-ray structure of TEAD4(Y429H) mutant) complexed with YAP (wildtype): Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson's chorioretinal atrophy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.99981 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 58.843, 58.843, 159.101 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.950 - 1.650 |
R-factor | 0.2252 |
Rwork | 0.225 |
R-free | 0.23730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6ge3 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.023 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.950 | 1.690 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.079 | 1.010 |
Rmeas | 0.083 | 1.050 |
Rpim | 0.880 | |
Total number of observations | 449159 | |
Number of reflections | 34599 | |
<I/σ(I)> | 21 | 2.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 13 | 13.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 1.4M Na/K PO4 |