6HEI

Structure of the catalytic domain of USP28 (insertion deleted) bound to Ubiquitin-PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 2 21 21
Unit cell lengths	49.505, 86.414, 97.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.510 - 1.640
R-factor	0.1871
Rwork	0.186
R-free	0.21380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nbf
RMSD bond length	0.007
RMSD bond angle	0.997
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	1.700
High resolution limit [Å]	1.640	1.640
Rmerge	0.049	0.637
Rmeas	0.057	0.733
Number of reflections	52145	5114
<I/σ(I)>	13.5	2.3
Completeness [%]	99.8	99.5
Redundancy	4	4
CC(1/2)	0.998	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	22% (w/v) PEG 3350, 300 mM potassium sodium tartrate