6HDP
Human DYRK2 bound to Scorzodihydrostilbene A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 84.527, 84.527, 148.372 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.820 - 2.300 |
| R-factor | 0.18635 |
| Rwork | 0.184 |
| R-free | 0.23776 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4azf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.198 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.530 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.093 | 0.837 |
| Number of reflections | 24654 | 3523 |
| <I/σ(I)> | 14.7 | 2.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.15M sodium malate and 20% PEG 3350 |
