6HC3
TFAM bound to Site-X
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-10 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.87260 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 164.790, 145.136, 108.119 |
| Unit cell angles | 90.00, 130.83, 90.00 |
Refinement procedure
| Resolution | 42.210 - 3.100 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.22930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tq6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.380 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.210 | 42.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.076 | |
| Number of reflections | 34552 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 18-24% PEG 3350, sodium potassium tartrate 0.1-0.25M |
