6H8P

JMJD2A/ KDM4A COMPLEXED WITH NI(II), NOG AND Histone H1.4(18-32)K26me3 peptide (15-mer)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.91741
Spacegroup name	P 21 21 2
Unit cell lengths	100.722, 150.146, 57.601
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.440 - 1.983
R-factor	0.193
Rwork	0.192
R-free	0.21180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ox0
RMSD bond length	0.005
RMSD bond angle	0.886
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.450	2.050
High resolution limit [Å]	1.980	1.980
Rmerge	0.083	0.937
Number of reflections	60704	5991
<I/σ(I)>	26.5	2.5
Completeness [%]	99.1	99.4
Redundancy	8.1	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	277	0.1 M MIB buffer (malonic acid/imidazole/boric acid, pH 6.0) 25% w/v polyethylene glycol 1500