6H78
E1 enzyme for ubiquitin like protein activation.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-12 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 143.860, 151.933, 153.543 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 107.980 - 2.700 |
| R-factor | 0.19014 |
| Rwork | 0.190 |
| R-free | 0.24224 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h8v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.493 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.980 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.176 | 1.447 |
| Number of reflections | 156266 | 7783 |
| <I/σ(I)> | 5.1 | 0.8 |
| Completeness [%] | 87.6 | 87.6 |
| Redundancy | 2.7 | 2.6 |
| CC(1/2) | 0.979 | 0.163 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293.13 | 0.2M sodium citrate tribasic dihydrate pH 7.9, 20% PEG 3350. |
