6H0P
The structure of C100A mutant of Arabidopsis thaliana UDP-apiose/UDP-xylose synthase in complex with NADH and UDP-D-glucuronic acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-20 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.000040 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 144.564, 144.564, 130.519 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.440 - 3.470 |
R-factor | 0.19806 |
Rwork | 0.196 |
R-free | 0.23904 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6h0n |
RMSD bond length | 0.015 |
RMSD bond angle | 2.230 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.440 | 3.800 |
High resolution limit [Å] | 3.470 | 3.470 |
Rmerge | 0.151 | 0.800 |
Number of reflections | 20833 | 4867 |
<I/σ(I)> | 13.1 | 1.9 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 8.3 | 7.9 |
CC(1/2) | 0.998 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.5 | 298 | 0.8 M sodium phosphate, 0.8 M potassium phosphate, and 0.1 M sodium-HEPES (pH 7.5) |