6GO5
TdT chimera (Loop1 of pol mu) - Ternary complex with 1-nt gapped DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 234.180, 69.250, 59.690 |
| Unit cell angles | 90.00, 95.20, 90.00 |
Refinement procedure
| Resolution | 44.860 - 2.350 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.26500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.860 | 2.490 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.065 | 0.813 |
| Number of reflections | 39224 | 6269 |
| <I/σ(I)> | 10.54 | |
| Completeness [%] | 98.2 | 98 |
| Redundancy | 2.8 | |
| CC(1/2) | 0.998 | 0.575 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 19-25% PEG 4000, 100-400 mM lithium sulfate, 100 mM tris |
