6GMR
pVHL:EloB:EloC in complex with (4-(1H-pyrrol-1-yl)phenyl)methanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07169 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.880, 59.880, 246.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.843 - 1.750 |
| R-factor | 0.2122 |
| Rwork | 0.211 |
| R-free | 0.23550 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.844 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | BUSTER-TNT |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 45106 | |
| <I/σ(I)> | 28.69 | 2.48 |
| Completeness [%] | 96.5 | 99.2 |
| Redundancy | 12.1 | 12.5 |
| CC(1/2) | 1.000 | 0.865 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M K phosphate pH 6.6, 0.2 M AS, 20% PEG MME 5000, 5% DTT |
