6GMN

pVHL:EloB:EloC in complex with methyl 4H-furo[3,2-b]pyrrole-5-carboxylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8856
Spacegroup name	P 41 2 2
Unit cell lengths	93.710, 93.710, 364.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.470 - 1.940
R-factor	0.2048
Rwork	0.203
R-free	0.24390
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.009
RMSD bond angle	1.155
Data reduction software	XDS
Data scaling software	XDS
Phasing software	BUSTER-TNT
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.060
High resolution limit [Å]	1.940	1.940
Rmeas	0.077	0.798
Number of reflections	226893	36190
<I/σ(I)>	13.75	2.4
Completeness [%]	99.4	98.1
Redundancy	7	7
CC(1/2)	0.998	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		285	0.1 M Na cacodylate pH 6.0, 0.2 M Mg acetate, 15% PEG3350, 5mM DTT