6GKU
Structure of galectin-10 in complex with the Fab fragment of a Charcot-Leyden crystal solubilizing antibody, 6F5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-27 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.87313 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 59.014, 146.186, 185.697 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.020 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmeas | 0.240 | 2.150 |
Number of reflections | 63019 | 9927 |
<I/σ(I)> | 7.67 | 1 |
Completeness [%] | 99.7 | 98.3 |
Redundancy | 12.8 | 11.4 |
CC(1/2) | 0.995 | 0.400 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.04 M calcium chloride 0.04 M sodium formate 0.1 M PIPES pH 7.0 8% PEG Smear High |