6GFF
Structure of GARP (LRRC32) in complex with latent TGF-beta1 and MHG-8 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.860, 175.010, 145.770 |
| Unit cell angles | 90.00, 92.23, 90.00 |
Refinement procedure
| Resolution | 29.921 - 3.100 |
| R-factor | 0.2348 |
| Rwork | 0.233 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.548 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.180 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 84967 | 6320 |
| <I/σ(I)> | 8.15 | |
| Completeness [%] | 90.1 | 90.6 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 15% (w/v) PEG4000, 0.1 M HEPES pH 6.75 |
