6GEK

TEAD4 (216-434);Y429F COMPLEXED WITH YAP PEPTIDE (60-100) AND MYRISTOATE (COVALENTLY BOUND) AT 2.28A (P212121 CRYSTAL FORM); MYRISTOYLATION WAS DONE BY ADDING MYR-COA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	P 21 21 21
Unit cell lengths	43.236, 77.041, 164.033
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 - 2.280
R-factor	0.2209
Rwork	0.220
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ge3
RMSD bond length	0.007
RMSD bond angle	0.951
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.111	0.651
Rmeas	0.121	0.704
Total number of observations	163458
Number of reflections	25747
<I/σ(I)>	12.1	2.7
Completeness [%]	99.7	100
Redundancy	6.3	6.9
CC(1/2)	0.997	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	25% PEG 3350, 0.2M MgCl2, 0.1M Bis-TRIS