6GDY
Crystal structure of 2OG oxygenase JMJD6 (aa 1-343) in complex with Fe(II) and 2OG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.07206 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.150, 96.990, 93.760 |
| Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
| Resolution | 42.227 - 2.040 |
| R-factor | 0.1812 |
| Rwork | 0.179 |
| R-free | 0.21620 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.583 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((dev_2906: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.227 | 9.120 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.130 | 1.297 |
| Rmeas | 0.157 | 1.532 |
| Rpim | 0.060 | 0.590 |
| Number of reflections | 53266 | 3866 |
| <I/σ(I)> | 9.7 | 1.4 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 6.7 | 6.6 |
| CC(1/2) | 0.997 | 0.569 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | 0.1M HEPES pH 7.8, 0.7M NaH2PO4, 0.9M KH2PO4, 3% glycerol |
